The invasin gimB (genetic island associated with humannewbornmeningitis) is usually found in ExPEC (Extraintestinal Pathogenic Escherichia coli) such as UPEC (uropathogenic E. coli), NMEC (neonatal meningitisE. coli) and APEC (avian pathogenic E. coli). In NMEC, gimB is associated with the invasion process of the host cells. Due to the importance of E. coli as a zoonotic agent and the scarce information about the frequency of gimB-carryingstrains in different animal species, the aim of this study was to investigate the presence of gimB in isolates from bovine, swine, canine and feline clinical samples. PCR was conducted on 196 isolates and the identity of the amplicons was confirmed by sequencing. Of the samples tested, only E. coli SB278/94 from a bovine specimen was positive (1/47) for gimB, which represents 2.1% of the bovine isolates. The ability of SB278/94 to adhere to and invade eukaryotic cells was confirmed by adherence and gentamicin-protection assays using HeLa cells. This is the first study that investigates for gimB in bovine, canine and feline E. coli isolates and shows E. coli from the intestinal-bovine samples harboring gimB.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Swine Diseases
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Bacterial Adhesion
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Cat Diseases
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Cattle Diseases
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Escherichia coli Proteins
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Virulence Factors
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Dog Diseases
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Escherichia coli
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Intestines
Limits:
Animals
/
Humans
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2015
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Santa Maria/BR
Consulta Detalhada
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