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Dynamic expression pattern of kinesin accessory protein in Drosophila.
J Biosci ; 2002 Sep; 27(5): 479-87
Article in English | IMSEAR | ID: sea-110668
ABSTRACT
We have identified the Drosophila homologue of the non-motor accessory subunit of kinesin-II motor complex. It is homologous to the SpKAP115 of the sea urchin, KAP3A and KAP3B of the mouse, and SMAP protein in humans. In situ hybridization using a DmKAP specific cRNA probe has revealed a dynamic pattern of expression in the developing nervous system. The staining first appears in a subset of cells in the embryonic central nervous system at stage 13 and continues till the first instar larva stage. At the third instar larva stage the staining gets restricted to a few cells in the optic lobe and in the ventral ganglion region. It has also stained a subset of sensory neurons from late stage 13 and till the first instar larva stage. The DmKAP expression pattern in the nervous system corresponds well with that of Klp64D and Klp68D as reported earlier. In addition, we have found that the DmKAP gene is constitutively expressed in the germline cells and in follicle cells during oogenesis. These cells are also stained using an antibody to KLP68D protein, but mRNA in situ hybridization using KLP64D specific probe has not stained these cells. Together these results proved a basis for further analysis of tissue specific function of DmKAP in future.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Female / RNA, Messenger / RNA Probes / Central Nervous System / Kinesins / In Situ Hybridization / Larva / Animals Language: English Journal: J Biosci Year: 2002 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Female / RNA, Messenger / RNA Probes / Central Nervous System / Kinesins / In Situ Hybridization / Larva / Animals Language: English Journal: J Biosci Year: 2002 Type: Article