Immunophenotypic and intracellular cytokine profile of Indian patients with tuberculosis with and without human immunodeficiency virus co-infection.
Article
in English
| IMSEAR
| ID: sea-138748
ABSTRACT
Background. Tuberculosis (TB) occurs in more than 50% of human immunodeficiency virus (HIV) infected Indian patients. This study was carried out to determine the immunophenotypic and intracellular cytokine profile of patients with HIV-TB co-infection. Patients and Methods. Fifteen patients with HIV-TB co-infection and 15 each with TB alone and healthy individuals were studied. Immunophenotypic analysis and intracellular cytokines were measured using appropriate antibodies on a flowcytometer. Results. Percentage of CD3+ did not differ significantly in the three groups. The ratio of CD4+ CD8+ was reversed among patients with TB and HIV-TB. CD19+ and CD25+ were present on fewer cells of healthy individuals but this was not statistically significant. Significantly higher percentage of cells of patients with TB and HIV-TB were CD69 positive. Interferongamma (INF-g ) and tumour necrosis factor-alpha (TNF-a) levels are significantly reduced in the CD4+ cells of patients with HIV-TB when compared with those with TB and healthy individuals. In CD8+ cells of patients with HIV-TB, levels of TNF-a are higher when compared with the other two groups. Interleukin-2 (IL-2) producing cells were not significantly different in any of the above subsets. Monocytes in individuals with HIV-TB had significantly higher interleukin-6 (IL-6) and TNF-a. Conclusions. T-helper cells among patients with HIV-TB have significantly lower cytokine production. T-suppressor cells and monocytes produce more TNF-a. These findings may be significant in view of recent attempts to treat HIV-TB coinfected patients with anti-TNF therapy.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Prognosis
/
Tuberculosis
/
Humans
/
Male
/
Incidence
/
Prevalence
/
Cytokines
/
Immunophenotyping
/
CD4-CD8 Ratio
/
AIDS-Related Opportunistic Infections
Type of study:
Incidence study
/
Prevalence study
/
Prognostic study
Country/Region as subject:
Asia
Language:
English
Year:
2009
Type:
Article
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