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Immunohistochemical Study of Matrix Metalloproteinase-9 in Medial Collateral Ligament Epiligament in Rat Knee after Grade III Injury.
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-183465
ABSTRACT

BACKGROUND:

In the present study, we conduct an immunohistochemical analysis to evaluate the expression of matrix metalloproteinase-9 (MMP-9) in the epiligament (EL) after grade III injury of the medial collateral ligament (MCL) in an experimental model of a rat knee.

METHODS:

Twelve 8-month old male Wistar rats were used in this study. Three animals were used as controls, while the remaining nine underwent grade III injury of the MCL. The MMP-9 immunoreactivity was evaluated on the 8th, 16th and 30th day after injury.

RESULTS:

We observed an intensive expression of the enzyme in all periods after injury in contrast with the control group. We also discovered that the main source of matrix metalloproteinase-9 was localized in the epiligament tissue. Immunoreactivity was highest and homogeneously distributed on the 8th day and gradually diminished, concentrating on the EL-ligament border and the perivascular zones on the 30th day.

CONCLUSION:

We present the first immunohistochemical study of the expression and distribution of the enzyme MMP-9 in the EL of the MCL and track the changes in enzyme activity on the 8th, 16th and 30th day after damage.

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Full text: Available Index: GHL / IMSEAR (South-East Asia) Language: English Year: 2017 Type: Article