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Melanocyte spheroids are formed by repetitive long-term trypsinization
Indian J Dermatol Venereol Leprol ; 2019 May; 85(3): 258-265
Article | IMSEAR | ID: sea-192490
ABSTRACT

Background:

Autologous melanocyte transplantation plays an important role in the treatment of vitiligo.

Objective:

Previous studies have indicated that, compared with melanocytes growing in monolayers, melanocyte spheroids have a better survival in growth factor- and serum-deprived conditions.

Methods:

Melanocyte spheroids were obtained from human epidermis by repetitive long-term trypsinization and maintained an aggregated morphology for a short period in certain conditions.

Results:

Melanocyte spheroids were capable of growing into normal dendritic melanocytes in monolayer when they were harvested and reinoculated in 24-well plates. Immunohistochemical analysis of the melanocyte spheroids revealed that they were positive for HMB45, a melanosome-specific marker. No melanomas occurred when melanocyte spheroids were transplanted into mice.

Conclusion:

Our study provides a promising approach for melanocyte transplantation to treat vitiligo.

Full text: Available Index: IMSEAR (South-East Asia) Journal: Indian J Dermatol Venereol Leprol Year: 2019 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Journal: Indian J Dermatol Venereol Leprol Year: 2019 Type: Article