Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design.
Indian J Biochem Biophys
;
2008 Dec; 45(6): 404-9
Article
in English
| IMSEAR
| ID: sea-26547
ABSTRACT
The improvement of xylanase production by Sclerotinia sclerotiorum S2 using a liquid fermentation culture was investigated. The optimized process was divided into three basic steps:
(i) evaluating xylanase inducers using different agricultural residues such as wheat bran, oat bran, orange peel and barley bran at 1% final concentration, and also filter paper. Among these, wheat bran showed the maximum activity (2.5 U/ml) at 12 days post-inoculation; (ii) for optimization, we determined the optimal concentration of inducer, the effect of phosphate anion (K2HPO4/KH2PO4) and culture aeration using a rotary shaker at 100 and 180 rpm. The optimal conditions for these three factors were determined in an experimental panel using factorial data, in which a mathematical model (Minitab software) was fitted; (iii) The optimized culture medium containing a high level of wheat bran (3%) without KH2PO4-K2HPO4 and submitted to a high agitation (180 rpm/min) increased the xylanase production from 2.5 U/ml to 4 U/ml (1.6-fold).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phosphates
/
Ascomycota
/
Hordeum
/
Dietary Fiber
/
Statistics as Topic
/
Bioreactors
/
Culture Media
/
Endo-1,4-beta Xylanases
/
Extracellular Space
/
Fermentation
Type of study:
Prognostic study
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2008
Type:
Article
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