A new membrane probing steroidal spin label: synthesis and applications.
Indian J Biochem Biophys
;
2000 Feb; 37(1): 45-50
Article
in English
| IMSEAR
| ID: sea-27850
ABSTRACT
The applicability of a new steroidal spin label, 3-oxo-androstan-17 beta-yl-(2",2",6",6"-tetramethyl-N-oxyl) piperidyl butan-1',4'-dioate, in studying the phase transition properties of model membrane L-alpha-dipalmitoyl phosphatidyl choline (DPPC) in the presence and absence of drugs has been explored. Its synthesis and characterization has been described herein. Besides, the localization of this spin label in lipid liposomes has been studied using electron spin resonance (ESR), differential scanning calorimetry (DSC) and 1H and 31P NMR spectroscopic techniques. The label has also been used to study the permeability of epinephrine into membrane. The results show that the spin label has a good potential as a spin probe in the study of biomembranes.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Spin Labels
/
1,2-Dipalmitoylphosphatidylcholine
/
Magnetic Resonance Spectroscopy
/
Electron Spin Resonance Spectroscopy
/
Androstanes
/
Lipid Bilayers
/
Membranes, Artificial
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2000
Type:
Article
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