Cloning and sequencing of beta toxin gene of Clostridium perfringens type C.
Indian J Exp Biol
;
2002 Jan; 40(1): 109-10
Article
in English
| IMSEAR
| ID: sea-56609
ABSTRACT
A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacterial Toxins
/
DNA, Bacterial
/
Molecular Sequence Data
/
Restriction Mapping
/
Blotting, Southern
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
Clostridium perfringens
/
Sequence Analysis, DNA
/
DNA Primers
Language:
English
Journal:
Indian J Exp Biol
Year:
2002
Type:
Article
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