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Cloning and sequencing of beta toxin gene of Clostridium perfringens type C.
Indian J Exp Biol ; 2002 Jan; 40(1): 109-10
Article in English | IMSEAR | ID: sea-56609
ABSTRACT
A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Bacterial Toxins / DNA, Bacterial / Molecular Sequence Data / Restriction Mapping / Blotting, Southern / Polymerase Chain Reaction / Cloning, Molecular / Clostridium perfringens / Sequence Analysis, DNA / DNA Primers Language: English Journal: Indian J Exp Biol Year: 2002 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Bacterial Toxins / DNA, Bacterial / Molecular Sequence Data / Restriction Mapping / Blotting, Southern / Polymerase Chain Reaction / Cloning, Molecular / Clostridium perfringens / Sequence Analysis, DNA / DNA Primers Language: English Journal: Indian J Exp Biol Year: 2002 Type: Article