Evaluation of IH-1000 for Automated ABO-Rh Typing and Irregular Antibody Screening / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 127-135, 2012.
Article
in Ko
| WPRIM
| ID: wpr-101151
Responsible library:
WPRO
ABSTRACT
BACKGROUND: Despite modern advances in laboratory automated medicine, work-process in the blood bank is still handled manually. Several automated immunohematological instruments have been developed and are available in the market. The IH-1000 (Bio-Rad Laboratories, Hercules, CA, USA), a fully automated instrument for immunohematology, was recently introduced. In this study, we evaluated the performance of the IH-1000 for ABO/Rh typing and irregular antibody screening. METHODS: In October 2011, a total of 373 blood samples for ABO/Rh typing and 303 cases for unexpected antibody screening were collected. The IH-1000 was compared to the manual tube and slide methods for ABO/Rh typing and to the microcolumn agglutination method (DiaMed-ID system) for antibody screening. RESULTS: For ABO/Rh typing, concordance rate was 100%. For unexpected antibody screening, positive results for both column agglutination and IH-1000 were observed in 10 cases (four cases of anti-E and c, three of anti-E, one of anti-D, one of anti-M, and one of anti-Xg) and negative results for both were observed in 289 cases. The concordance rate between IH-1000 and column agglutination was 98.7%. Sensitivity and specificity were 90.9% and 99.3%, respectively. CONCLUSION: The automated IH-1000 showed good correlation with the manual tube and slide methods and the microcolumn agglutination method for ABO-RhD typing and irregular antibody screening. The IH-1000 can be used for routine pre-transfusion testing in the blood bank.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Automation
/
Blood Banks
/
Mass Screening
/
Sensitivity and Specificity
/
Agglutination
/
Isoantibodies
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
Ko
Journal:
Korean Journal of Blood Transfusion
Year:
2012
Type:
Article