Association between polymorphisms of gamma-aminobutyric acid B receptor 1 gene and prodynorphin promoter region gene and epilepsy secondary to cerebral infarction / 中华神经医学杂志

ABSTRACT

Objective To investigate the relationship between the polymorphisms of gamma-aminobutyric acid B receptor 1 (GABABR1) gene (G1465A) and prodynorphin (PDYN) promoter region gene and epilepsy secondary to cerebral infarction in Han population.Methods Sixty-seven patients with epilepsy secondary to cerebral infarction,93 patients diagnosed as having cerebral infarction without epilepsy and 104 healthy volunteers were selected in our hospitals from July 2010 to August 2011.EDTA-blood specimens were collected from test objects.DNA samples were extracted with column chromatography.The polymorphism of GABABR1 (G1465A) gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique,and the polymorphism of PDYN gene promoter region was detected by PCR technique.Results Only genotype G/G was observed in the three groups without noting genotypes A/G and A/A in our study.The frequency of L/L genotype in patients with epilepsy secondary to cerebral infarction (76.1％) was slightly higher than that in patients with cerebral infarction without epilepsy (73.2％) and normal control group (73.1％) without significant difference (P＞0.05).The frequency of L allele in patients with epilepsy secondary to cerebral infarction,patients with cerebral infarction without epilepsy and normal control group was 85.8％,85.5％ and 84.6％,respectively,without significant difference (P＞0.05).Conclusion There may be no association between the polymorphisms of GABABR1 (G 1465A) gene and PDYN promoter region gene and epilepsy secondary to cerebral infarction in Han population.