In silico Prediction of Angiogenesis-related Genes in Human Hepatocellular Carcinoma
Genomics & Informatics
;
: 134-141, 2004.
Article
in English
| WPRIM
| ID: wpr-105279
ABSTRACT
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and a typical hypervascular tumor. Therefore, it is important to find factors related to angiogenesis in the process of HCC malignancy. In order to find angiogenesis-related factors in HCC, we used combined methods of in silico prediction and an experimental assay. We analyzed 1457 genes extracted from cDNA microarray of HCC patients by text-mining, sequence similarity search and domain analysis. As a result, we predicted that 16 genes were likely to be involved in angiogenesis and then the effects of these genes were confirmed by hypoxia response element(HRE)-luciferase assay. For instant,we classified osteopontin into a potent angiogenic factor and coagulation factor XII into a significant anti-angiogenic factor. Collectively, we suggest that using a combination of in silico prediction and experimental approaches, we can identify HCC-specific angiogenesis-related factors effectively and rapidly.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Computer Simulation
/
Factor XII
/
Carcinoma, Hepatocellular
/
Computational Biology
/
Oligonucleotide Array Sequence Analysis
/
Angiogenesis Inducing Agents
/
Osteopontin
/
Hypoxia
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Genomics & Informatics
Year:
2004
Type:
Article
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