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In silico Prediction of Angiogenesis-related Genes in Human Hepatocellular Carcinoma
Genomics & Informatics ; : 134-141, 2004.
Article in English | WPRIM | ID: wpr-105279
ABSTRACT
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and a typical hypervascular tumor. Therefore, it is important to find factors related to angiogenesis in the process of HCC malignancy. In order to find angiogenesis-related factors in HCC, we used combined methods of in silico prediction and an experimental assay. We analyzed 1457 genes extracted from cDNA microarray of HCC patients by text-mining, sequence similarity search and domain analysis. As a result, we predicted that 16 genes were likely to be involved in angiogenesis and then the effects of these genes were confirmed by hypoxia response element(HRE)-luciferase assay. For instant,we classified osteopontin into a potent angiogenic factor and coagulation factor XII into a significant anti-angiogenic factor. Collectively, we suggest that using a combination of in silico prediction and experimental approaches, we can identify HCC-specific angiogenesis-related factors effectively and rapidly.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Computer Simulation / Factor XII / Carcinoma, Hepatocellular / Computational Biology / Oligonucleotide Array Sequence Analysis / Angiogenesis Inducing Agents / Osteopontin / Hypoxia Type of study: Prognostic study Limits: Humans Language: English Journal: Genomics & Informatics Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Computer Simulation / Factor XII / Carcinoma, Hepatocellular / Computational Biology / Oligonucleotide Array Sequence Analysis / Angiogenesis Inducing Agents / Osteopontin / Hypoxia Type of study: Prognostic study Limits: Humans Language: English Journal: Genomics & Informatics Year: 2004 Type: Article