Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair
Journal of Veterinary Science
;
: 295-297, 2011.
Article
in English
| WPRIM
| ID: wpr-108422
ABSTRACT
The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bovine Virus Diarrhea-Mucosal Disease
/
RNA, Viral
/
Diarrhea Virus 1, Bovine Viral
/
Diarrhea Virus 2, Bovine Viral
/
Real-Time Polymerase Chain Reaction
/
Hair
/
Antibodies, Viral
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2011
Type:
Article
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