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Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair
Journal of Veterinary Science ; : 295-297, 2011.
Article in English | WPRIM | ID: wpr-108422
ABSTRACT
The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Bovine Virus Diarrhea-Mucosal Disease / RNA, Viral / Diarrhea Virus 1, Bovine Viral / Diarrhea Virus 2, Bovine Viral / Real-Time Polymerase Chain Reaction / Hair / Antibodies, Viral Type of study: Diagnostic study Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bovine Virus Diarrhea-Mucosal Disease / RNA, Viral / Diarrhea Virus 1, Bovine Viral / Diarrhea Virus 2, Bovine Viral / Real-Time Polymerase Chain Reaction / Hair / Antibodies, Viral Type of study: Diagnostic study Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2011 Type: Article