Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Journal of Veterinary Science
;
: 253-256, 2017.
Article
in English
| WPRIM
| ID: wpr-109772
ABSTRACT
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10(1.5) TCID₅₀/mL for type 2 PRRSV and 10² infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of agreement. This RNA-based duplex RT-PCR approach can be another way to detect type 2 PRRSV and PCV2 simultaneously and with improved convenience.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA
/
RNA, Messenger
/
RNA-Directed DNA Polymerase
/
Circovirus
/
Porcine respiratory and reproductive syndrome virus
/
Porcine Reproductive and Respiratory Syndrome
/
Reverse Transcriptase Polymerase Chain Reaction
/
Limit of Detection
/
Methods
Type of study:
Diagnostic study
Language:
English
Journal:
Journal of Veterinary Science
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS