Platelets Induce Proliferation of Human Umbilical Vein Endothelial Cells via CD154-CD40 Pathway Independently of VEGF
Immune Network
;
: 75-81, 2008.
Article
in English
| WPRIM
| ID: wpr-112843
ABSTRACT
BACKGROUND:
Platelets take part in repairing the lesions of endothelial damage. To understand the molecular mechanism of this process, we tested the hypothesis that CD154 expressed on activated platelets stimulates proliferation of human endothelial cells.METHODS:
The expression levels of CD154 and CD40 on platelets and endothelial cells, respectively, were measured by flow cytometry and confocal microscopy. Function-blocking monoclonal antibody against CD154 was developed after immunization with CD154- transfected L cells.RESULTS:
An anti-CD40 agonist antibody and soluble CD154 both induced significant proliferation of endothelial cells. In addition, a function-blocking anti-CD154 antibody inhibited the platelet-induced proliferation of endothelial cells, indicating that the CD154-CD40 pathway is involved in these cellular interactions. An anti-VEGF antibody failed to inhibit the proliferation. This, in addition to the fact that very small amounts of VEGF are released from platelets or endothelial cells, suggests that VEGF does not play an important role in the platelet-stimulated proliferation of endothelial cells.CONCLUSION:
Our results indicate that platelets induce proliferation of endothelial cells by CD154-CD40 interactions independently of VEGF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood Platelets
/
Immunization
/
Microscopy, Confocal
/
Endothelial Cells
/
Vascular Endothelial Growth Factor A
/
Human Umbilical Vein Endothelial Cells
/
Flow Cytometry
Limits:
Humans
Language:
English
Journal:
Immune Network
Year:
2008
Type:
Article
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