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Relationship between meiotic spindles visualization and intracytoplasmic sperm injection outcomes in human oocytes / 대한해부학회지
Anatomy & Cell Biology ; : 26-32, 2017.
Article in English | WPRIM | ID: wpr-193191
ABSTRACT
In assisted reproductive techniques, the operator attempts to select morphologically best embryos to predict embryo viability. Development of polarized light microscope, which evaluates the oocytes' spindles according to birefringence of living cells, had been helpful in oocyte selection. The aim of this study is evaluating the relationship between meiotic spindles visualization and intracytoplasmic sperm injection (ICSI) outcomes in human oocytes. In this study, 264 oocytes from 24 patients with an average age of 30.5±7.5 years with infertility duration of 1 to 10 years were collected. The oocytes were randomly allocated to the control injection group (n=126) and the oocyte imaging group (spindle-aligned group) (n=138). In the spindle-aligned group, the meiotic spindle was identified by means of polarized light microscope to align the spindle at 6 or 12 o'clock. Then the spindle-aligned group was divided into three sub-groups based on spindle morphology fine, average, and (poor). After ICSI, embryos were checked every 24 hours and scored; 72 hours later, high-grade embryos were transferred intravaginally to uterus. This study showed that the fertilization rate in the spindle-aligned group was higher than the control group (P<0.05). After cleavage, a positive correlation was observed between spindle morphology and embryo morphology. Among the sub-groups of spindle-aligned group, the embryos' morphology of the fine group was better than the other subgroups and embryos of the poor group had lower quality and more fragmentation. The results revealed that the selection of oocytes based on meiotic spindle imaging can significantly improve the rate of fertilization and embryo cleavage and certainly increase the rate of implantation.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oocytes / Uterus / Birefringence / Sperm Injections, Intracytoplasmic / Reproductive Techniques, Assisted / Embryonic Structures / Fertilization / Infertility / Microscopy, Polarization / Spindle Apparatus Type of study: Prognostic study Limits: Humans Language: English Journal: Anatomy & Cell Biology Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oocytes / Uterus / Birefringence / Sperm Injections, Intracytoplasmic / Reproductive Techniques, Assisted / Embryonic Structures / Fertilization / Infertility / Microscopy, Polarization / Spindle Apparatus Type of study: Prognostic study Limits: Humans Language: English Journal: Anatomy & Cell Biology Year: 2017 Type: Article