NF-kappaB Binding Activity and Cyclooxygenase-2 Expression in Persistent betaCCI(4)-Treated Rat Liver Injury
Journal of Korean Medical Science
; : 193-200, 2002.
Article
in En
| WPRIM
| ID: wpr-197892
Responsible library:
WPRO
ABSTRACT
The involvement of NF-kappaB binding activity is known to be important in the mechanism of acute liver injury and in the induction of cyclooxygenase (COX-2). This study was performed to evaluate NF-kappaB binding activity and the expression of COX-2 in chronic liver injury induced by carbon tetrachloride (betaCCI(4)). Liver tissues from Sprague - Dawley rats were collected at 1, 3, 5, and 7th week after intraperitoneal injection of 0.1 mL of betaCCI(4)/100 g body weight twice a week. Reactive oxy-gen species (ROS) were measured in the postmitochondrial fraction by dichlorofluorescein formation with a fluorescent probe. An electrophoretic mobility shift assay was performed for NF-kappaB binding activity. Western blot was performed to measure the level of COX-1, COX-2, p65, p50, and I B proteins. ROS and NF-kappaB activity increased during the CCl4-induced chronic liver injury. The expression of nuclear p65 protein and p50 protein increased compared with that of the control, while the cytoplasmic I B protein decreased as the inflammation persisted. The expression of COX-2 in betaCCI(4)-treated rat liver increased compared with that of the control. It could be suggested that ROS produced by betaCCI(4) treatment increased NF-kappaB binding activity and thereby COX-2 expression, and these might be implicated in the progress of chronic liver damage.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Protein Binding
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Biological Transport
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Carbon Tetrachloride
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Carbon Tetrachloride Poisoning
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Cell Nucleus
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NF-kappa B
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Prostaglandin-Endoperoxide Synthases
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Reactive Oxygen Species
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Rats, Sprague-Dawley
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I-kappa B Proteins
Limits:
Animals
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2002
Type:
Article