The association of follicular fluid volume with human oolemma stretchability during intracytoplasmic sperm injection / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 126-131, 2017.
Article
in English
| WPRIM
| ID: wpr-219266
ABSTRACT
OBJECTIVE:
Oocyte degeneration often occurs after intracytoplasmic sperm injection (ICSI), and the risk factor is low-quality oocytes. The follicular fluid (FF) provides a crucial microenvironment for oocyte development. We investigated the relationships between the FF volume aspirated from individual follicles and oocyte retrieval, oocyte maturity, oolemma stretchability, fertilization, and development.METHODS:
This retrospective study included data obtained from 229 ICSI cycles. Ovarian stimulation was performed according to a gonadotropin-releasing hormone antagonist protocol. Each follicle was individually aspirated and divided into six groups according to FF volume (<1.0, 1.0 to <2.0, 2.0 to <3.0, 3.0 to <4.0, 4.0 to <5.0, and ≥5.0 mL). Oolemma stretchability during ICSI was evaluated using a mechanical stimulus for oolemma penetration, that is, the stretchability was assessed by oolemma penetration with aspiration (high stretchability) or without aspiration (low stretchability).RESULTS:
Oocyte retrieval rates were significantly lower in the <1.0 mL group than in the ≥1.0 mL groups (46.0% [86/187] vs. 67.5%–74.3% [172/255 to 124/167], respectively; p<0.01). Low oolemma stretchability was significantly more common in the <1.0 mL group than in the ≥1.0 mL groups during ICSI (22.0% [13/59] vs. 5.8%–9.4% [6/104 to 13/139], respectively; p=0.018). There was a relationship between FF volume and oolemma stretchability. However, there were no significant differences in the rates of fertilization, cleavage, ≥7 cells at day 3, and blastocyst development among all groups.CONCLUSION:
FF volume is potentially associated with the stretchability of metaphase II oolemma during ICSI. Regarding oolemma stretchability, ensuring a uniform follicular size during ovarian stimulation is crucial to obtain good-quality oocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Ovulation Induction
/
Blastocyst
/
Follicular Fluid
/
Retrospective Studies
/
Risk Factors
/
Gonadotropin-Releasing Hormone
/
Clothing
/
Sperm Injections, Intracytoplasmic
/
Oocyte Retrieval
Type of study:
Etiology study
/
Observational study
/
Risk factors
Limits:
Female
/
Humans
Language:
English
Journal:
Clinical and Experimental Reproductive Medicine
Year:
2017
Type:
Article
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