Production of Stromal Cell-Derived Factor-1 (SDF-1)and Expression of CXCR4 in Human Bone Marrow Endothelial Cells
Journal of Korean Medical Science
; : 679-685, 2003.
Article
in En
| WPRIM
| ID: wpr-221856
Responsible library:
WPRO
ABSTRACT
This study investigated the production of stromal cell-derived factor-1 (SDF-1) and the expression of CXCR4 in human bone marrow endothelial cells (BMECs). Human BMEC cell line BMEC-1 cells expressed SDF-1 mRNA, and conditioned medium induced chemoattraction of CD34+ cells. Migration was not inhibited by pretreating the input cells with pertussis toxin, indicating that the chemoattractive activity was not dependent on SDF-1. Three-day culture of BMEC-1 and primary human BMEC cells produced 1,710+/-204 and 1,050+/-153 pg/mL SDF-1alpha, respectively, which was much less than primary human BM stromal cells (29,536+/-532 pg/ mL). By immuno-histochemistry, CXCR4 was detected in the endothelial cells lining sinusoids, arterioles, and venules in the bone marrow. However, cultured BMECs and BMEC-1 cells did not express CXCR4 on their surfaces. These results indicate that BMECs produce and release small amounts of SDF-1 and express CXCR4 in vivo only.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Time Factors
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Umbilical Veins
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RNA, Messenger
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Bone Marrow Cells
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Enzyme-Linked Immunosorbent Assay
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Hematopoietic Stem Cells
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Immunohistochemistry
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Cell Movement
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Cells, Cultured
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Chemotaxis
Limits:
Humans
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2003
Type:
Article