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Neural stem cells induced by neotype three-dimensional polypeptide-based self-assembled hydrogel / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 612-616, 2010.
Article in Chinese | WPRIM | ID: wpr-230819
ABSTRACT
The amphiphilic polypeptide (PA) was self-assembled into three-dimensional (3-D) porous complex of hydrogel and cells with the addition of NSCs-containing DMEM/F12. Cell differentiation in the surface and that within hydrogel were described. Cells harvested from the cerebral cortex of neonatal mice were triturated and cultivated in serum-free media. 1wt% PA was added into same volume of DMEM/F12 with cell concentration of 1 x 10(5)/ml and self-supported into 3-D hydrogel-cell composition; cells suspended within hydrogel being maintained (Experiment group, EG). lwt% PA was self-assembled into two-dimensional (2-D) hydrogel films triggered by addition of DMEM/F12, and then 1 x 10(5)/ml NSCs was seeded in the surface of films (Control group, CG). Cells in EG and CG were incubated in serum-free media for two weeks and stained with immunocytochemistry methods. TEM showed that the hydrogel derived from PA was composed of network nanofibers with their diameter ranging from 3 to 5 nm and length ranging from 100 nm to 1. 5 microm. Above 50% of cells obtained were Nestin positive cells. LSCM observations demonstrated that above 90% of cells survived two days after incubation within hydrogel, and were differentiated into NF and GFAP positive cells one week after incubation, their differentiation rates were 50% +/- 4.2% and 20% +/- 2.8% respectively; however, cells in CG were also differentiated into NF and GFAP positive cells, their differentiation rates were only 40% +/- 3.4% and 31% +/- 2.3% separately. Peptide-based hydrogel was able to provide 3-D environments for cell survival and induce primarily the differentiation of NSCs into neurons. Our data indicated that peptide-directed self-assembly of hydrogels was useful and it served as the neotype nerve tissue engineering scaffolds.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptides / Cell Differentiation / Cells, Cultured / Chemistry / Rats, Sprague-Dawley / Cell Culture Techniques / Hydrogel, Polyethylene Glycol Dimethacrylate / Cell Biology / Tissue Scaffolds / Neurogenesis Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptides / Cell Differentiation / Cells, Cultured / Chemistry / Rats, Sprague-Dawley / Cell Culture Techniques / Hydrogel, Polyethylene Glycol Dimethacrylate / Cell Biology / Tissue Scaffolds / Neurogenesis Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2010 Type: Article