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Effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca / 生物工程学报
Chinese Journal of Biotechnology ; (12): 350-357, 2013.
Article in Zh | WPRIM | ID: wpr-233239
Responsible library: WPRO
ABSTRACT
To get the tolerability and consumption of Klebsiella oxytoca on major inhibitors in lignocelluloses hydrolysate, we studied the effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca. The metabolites of furfural and 5-hydroxymethylfurfural were measured. The results show that when acetic acid, furfural and 5-hydroxymethylfurfural was individually added, tolerance threshold for Klebsiella oxytoca was 30 g/L, 4 g/L and 5 g/L, respectively. Acetic acid was likely used as substrate to produce 2,3-butanediol. The yield of 2,3-butanediol increased when acetic acid concentration was lower than 30 g/L. In the fermentation, more than 70% 5-hydroxymethylfurfural was converted to 2,5-furandimethanol. All furfural and the rest of 5-hydroxymethylfurfural were metabolized by Klebsiella oxytoca. It showed that in the detoxification process of 2,3-butanediol production using lignocelluloses hydrolysate, furfural should be given priority to remove and a certain concentration of acetic acid is not need to removal.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Butylene Glycols / Chemistry / Acetic Acid / Klebsiella oxytoca / Fermentation / Furaldehyde / Lignin / Metabolism Language: Zh Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article
Full text: 1 Index: WPRIM Main subject: Butylene Glycols / Chemistry / Acetic Acid / Klebsiella oxytoca / Fermentation / Furaldehyde / Lignin / Metabolism Language: Zh Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article