Genetic analysis of β -thalassemia mutations in the minority populations of Guizhou province / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 700-703, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-234332
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the gene mutation frequencies and patterns of β-thalassemia (β-thal) in the minority populations of Guizhou province.</p><p><b>METHODS</b>Three thousand and five hundred couples in the reproductive age were screened by using automatic hemocyte analyzer and hemoglobin autoanalyzer-variant. The diagnostic criteria for β-thal were the mean corpuscular volume (MCV) was ≤ 82 fl, and the HbA(2) level was ≥ 3.5%. A total of 194 positive samples were detected and further identified by PCR-reverse dot blot (PCR-RDB) assay for 18 common β -thal mutations in Chinese population. Those subjects with positive phenotypes but without the 18 common β-thal mutations were subjected to DNA sequence analysis of the β-globin gene.</p><p><b>RESULTS</b>One hundred and eighty-nine samples with gene mutations were observed from the 3500 samples, with the incidence of β-thal being 5.4%. A total of 10 different β-thal mutations were identified from the 189 diagnosed samples. The five most common mutations were as the following CD17 (43.9%), CD41-42 (38.6%), IVS-II-654(10.1%), -28 (2.6%) and CD71-72 (1.6%). In addition, a novel β-globin gene mutation (-CD53) allele was detected. One rare mutation of IntM was observed.</p><p><b>CONCLUSION</b>The minority population in Guizhou province is of high risk of β-thal. It is recommended that more attention should be paid to detect the carriers of β-thal in the population in reproductive age by hematologic screening and common gene diagnosis in the area with high risk of β-thal.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA Mutational Analysis
/
Base Sequence
/
Ethnicity
/
China
/
Beta-Thalassemia
/
Ethnology
/
Beta-Globins
/
Genetics
/
Mutation
Type of study:
Prognostic study
Limits:
Adult
/
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2010
Type:
Article
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