Effects of smoking on sperm apoptosis and semen quality of adult males in the main urban area of Chongqing / 中华男科学杂志
National Journal of Andrology
;
(12): 685-688, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-241277
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of smoking on sperm apoptosis and semen quality of healthy adult males in the main urban area of Chongqing.</p><p><b>METHODS</b>According to the smoking habit, we divided 235 healthy adult males into a non-smoking group (n = 89) and a smoking group (n = 146). Then we detected the routine semen parameters by the computer-assisted semen analysis system and obtained the parameters of sperm apoptosis (the ratios of AN-/PI-, AN+/PI-, AN+/PI+ and AN-/PI+ sperm) by flow cytometry combined with Annexin V-FITC/PI fluorescence staining.</p><p><b>RESULTS</b>The rate of early apoptotic sperm (AN+/PI-) was higher in the smoking than in the non-smoking group ([8.1 +/- 5.1]% vs [6.8 +/- 3.8]%; P = 0.039), but there were no significant differences between the two groups in the rate of late apoptotic sperm (AN+/PI+) ([5.6 +/- 5.2]% vs [5.5 +/- 5.1]%; P = 0.87), as well as in such routine semen indexes as semen volume, sperm density, sperm motility, sperm vitality and normal sperm morphology (P = 0.30, 0.82, 0.37, 0.81 and 0.84, respectively).</p><p><b>CONCLUSION</b>The rate of early apoptotic sperm is higher in smokers than in non-smokers, suggesting that smoking may induce early damage to sperm cells. Compared with routine semen parameters, sperm apoptosis is a more sensitive biomarker to reflect smoking-induced damage to sperm.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Semen
/
Sperm Count
/
Sperm Motility
/
Spermatozoa
/
Smoking
/
China
/
Apoptosis
/
Cell Biology
/
Semen Analysis
Limits:
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2009
Type:
Article
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