Effect of overexpressing isocitrate lyase on succinate production in ldh(-1) Corynebacterium glutamicum / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1696-1700, 2013.
Article
in Zh
| WPRIM
| ID: wpr-242421
Responsible library:
WPRO
ABSTRACT
Corynebacterium glutamicum SA001 is a mutant with lactate dehydrogenase (ldhA) deletion. In order to increase metabolic flux from isocitrate to succinate, and to improve the production of succinate under anaerobic conditions,we transducted the gene aceA coding isocitrate lyase (ICL) from Escherichia coli K12 into Corynebacterium glutamicum SA001 (SA001/pXMJ19-aceA). After 12 h aerobic induction by adding 0.8 mmol/L of IPTG, the recombinant strain was transferred to anaerobic fermentation for 16 h. Succinate reached 14.84 g/L, with a productivity of 0.83 g/(L x h). Compared to C. glutamicum SA001, the activity of ICL of the recombinant strain was increased 5.8-fold, and the succinate productivity was increased 48%. Overexpression of isocitrate lyase will increase the metabolic flux of glyoxylate bypass flowing to succinate.
Full text:
1
Index:
WPRIM
Main subject:
Transduction, Genetic
/
Industrial Microbiology
/
Gene Deletion
/
Succinic Acid
/
Corynebacterium glutamicum
/
Escherichia coli
/
Genetics
/
Isocitrate Lyase
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L-Lactate Dehydrogenase
/
Metabolism
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article