AcSDKP attenuates PDGF-induced cardiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 444-448, 2008.
Article
in Zh
| WPRIM
| ID: wpr-243760
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2 (ERK1/2) pathway on this process.</p><p><b>METHODS</b>Metabolic activity of fibroblasts was determined by CCK-8. Cell cycle was detected by flow cytometry. Expressions of type I and type III collagen were measured by immunocytochemistry and Western blot. Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot.</p><p><b>RESULT</b>10(-9) mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2, while the protein levels of ERK1/2 were not significantly affected by AcSDKP.</p><p><b>CONCLUSION</b>AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.</p>
Full text:
1
Index:
WPRIM
Main subject:
Oligopeptides
/
Phosphorylation
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Platelet-Derived Growth Factor
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Signal Transduction
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Cells, Cultured
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Collagen
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Rats, Wistar
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Mitogen-Activated Protein Kinase 1
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Cell Biology
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Myocytes, Cardiac
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Cardiology
Year:
2008
Type:
Article