Effect of anti-Helicobacter pylori ureB monoclonal antibody on platelet aggregation and activation, and its mechanism study / 中华血液学杂志
Chinese Journal of Hematology
; (12): 166-169, 2006.
Article
in Zh
| WPRIM
| ID: wpr-243977
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To study the effect of monoclonal antibody (McAb) against helicobacter pylori (Hp) ureB, 1F11 on platelet aggregation and activation, and its mechanism.</p><p><b>METHODS</b>The relativity between human platelet glycoproteins (GPs) and Hp ureB was identified by Western blot and FCM. Platelet aggregation was measured by turbidimetry, and P-selectin and TXB2 assay by ELISA.</p><p><b>RESULTS</b>1F11 could bind to platelet GPIIIa, and ADP-induced platelet aggregation was inhibited by 1F11 in a dose-dependent manner. However, 1F11 had no effect on plasma P-selectin and TXB2 induced by ADP. The FCM results show that the positive rates of platelet binding to FITC-SZ21 was decreased from 99.5% to 77.4% after addition of 1F11.</p><p><b>CONCLUSION</b>McAb against Hp ureB 1F11 inhibits platelet aggregation through binding to platelet GPIIIa but does not block platelet activation. There might be crossed-epitopes on Hp ureB and platelet GPIIIa, and Hp infection might be involved in ITP immunopathology.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Bacterial Proteins
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Urease
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Urokinase-Type Plasminogen Activator
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Platelet Activation
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Platelet Aggregation
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Helicobacter pylori
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P-Selectin
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Integrin beta3
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Allergy and Immunology
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2006
Type:
Article