Experimental study on the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats using proteomic approach.</p><p><b>METHODS</b>Thirty-seven rats were randomly divided into 3 groups, i. e., the normal control group (Group 1), the Pi-qi deficiency syndrome model group (Group 2), and the reserpine group (Group 3). The Pi-qi deficiency syndrome model was established using excessive exerting combined with irregular diet, and peritoneally injecting Reserpine Injection (1 mg/mL) respectively. The ileum tissues were separated after identified fuzzy method. The differentially expressed proteins were separated with two dimensional electrophoresis (2-DE), analyzed by Mass Spectrometry, and identified by MASCOT Software.</p><p><b>RESULTS</b>Three proteins were differentially expressed in Group 2 and nine proteins were differentially expressed in Group 3 (P < 0.05).</p><p><b>CONCLUSION</b>Pi-qi deficiency syndrome was closely related with decreased expression of albumin as well as increased expressions of trypsin and glucose-regulated protein 78.</p>