Rabbit urethral defect repair with freeze-dried acellular bladder submucosa / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 62-65, 2005.
Article
in Zh
| WPRIM
| ID: wpr-255102
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the result of urethral defect repair using freeze-dried acellular bladder submucosa.</p><p><b>METHODS</b>The freeze-dried acellular bladder submucosa was obtained from human cadaveric bladder submucosa using freeze-thawed enzymatic treatment and the freeze-drying technique. 18 male New Zealand rabbits were used in this experiment. A defect of 1.0 cm x 0.5 cm was created in the ventral urethra of the animal. The acellular matrix was grafted to the urethral defect in 14 rabbits. The other 4 animals underwent a sham operation as the controls. From postoperative one to twenty-four weeks, the animals underwent retrograde urethrography at certain intervals. The grafts of the acellular matrix were taken and prepared for histological evaluation.</p><p><b>RESULTS</b>No obvious urethral stricture was observed in the 14 matrix-grafted animals. Histological examinations of the specimens showed progressive infiltration of cells and vascular vessels. Complete epithelization was obtained at 2 weeks. The orientation of collagen fibers in the matrix-grafted area tended to be regular with time.</p><p><b>CONCLUSIONS</b>Freeze-dried acellular bladder submucosa may be a suitable material for urethral defect repair, which induces urethral mucous cells to migrate, grow and epithelialize.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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General Surgery
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Transplantation
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Urethra
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Urethral Stricture
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Urinary Bladder
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Plastic Surgery Procedures
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Cell Biology
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Tissue Engineering
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Extracellular Matrix
Limits:
Animals
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Plastic Surgery
Year:
2005
Type:
Article