In vitro anti-inflammatory effects of different solution fractions of ethanol extract from Melilotus suaveolens Ledeb / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1992-1998, 2007.
Article
in En
| WPRIM
| ID: wpr-255459
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Melilotus suaveolens Ledeb (M. suaveolens Ledeb) has long been used as a folk medicine in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant.</p><p><b>METHODS</b>Petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, aqueous fraction were obtained from ethanol extract of M. suaveolens Ledeb and evaluated by high performance liquid chromatography (HPLC). While dexamethasone (DM) was used as a positive control, the effects of different solution fractions of ethanol extract on tumor necrosis factor alpha (TNF-alpha) mRNA, cyclooxygenase 2 (COX-2) mRNA, COX-2 and nuclear factor kappaB (NF-kappaB) of LPS-stimulated RAW 264.7 cells were studied by real-time PCR, Western blot analysis and immunocytochemical assay, respectively.</p><p><b>RESULTS</b>Coumarin was one of the main ingredients in different solution fractions of ethanol extract except the aqueous fraction with no inflammatory effect. The petroleum ether fraction, ethyl acetate fraction and n-butanol fraction of ethanol extract could inhibit the production of TNF-alpha mRNA, COX-2 mRNA and NF-kappaB to some extent.</p><p><b>CONCLUSIONS</b>Different solution fractions of ethanol extract from M. suaveolens Ledeb had similar anti-inflammatory effect as did dexamethasone except the aqueous fraction. Coumarin was likely to be essential to the anti-inflammatory effect, and other ingredients might attribute to their different anti-inflammatory effects from the HPLC fingerprint.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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RNA, Messenger
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Plant Extracts
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Cell Line
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Chemistry
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Anti-Inflammatory Agents, Non-Steroidal
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Lipopolysaccharides
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NF-kappa B
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Tumor Necrosis Factor-alpha
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Melilotus
Limits:
Animals
Language:
En
Journal:
Chinese Medical Journal
Year:
2007
Type:
Article