Effects of ezrin silencing on pancreatic cancer cell line Panc-1 / 中华病理学杂志
Chinese Journal of Pathology
; (12): 833-836, 2012.
Article
in Zh
| WPRIM
| ID: wpr-256281
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.</p><p><b>METHODS</b>Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid. The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis, respectively. Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy. Colony formation assay was used to determine the cell anchor-independent growth ability in vitro. Trans-filter migration and invasion assays were performed with 8 µm pore inserts in a 24-well BioCoat chamber with/without Matrigel.</p><p><b>RESULTS</b>Ezrin silencing decreased cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion, but had no effects on cell proliferation in vitro and cell cycle, in pancreatic cancer cell line Panc-1.</p><p><b>CONCLUSION</b>Ezrin expression affects the cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion in pancreatic cancer cell line Panc-1.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pancreatic Neoplasms
/
Pathology
/
Plasmids
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Transfection
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Cell Movement
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Cell Surface Extensions
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Cytoskeletal Proteins
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RNA, Small Interfering
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RNA Interference
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Cell Line, Tumor
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2012
Type:
Article