Role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1456-1460, 2016.
Article
in Zh
| WPRIM
| ID: wpr-256577
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells and the mechanisms.</p><p><b>METHODS</b>MTT assay was used to assess the cytotoxicity of cisplatin (DDP) in I-10 cells. Annexin V/PI double staining and Hoechst 33258 fluorescence staining were employed to detect early- and late-stage apoptosis of the cells, respectively. Extracellular ATP level and intracellular IP3 level in the cells were detected using commercial detection kits.</p><p><b>RESULTS</b>I-10 cells exposed to both CBX (a pannexin 1 channel inhibitor) and DDP showed a higher cell viability compared with the cells exposed to DDP alone (P<0.01). CBX significantly decreased cisplatin-induced early-stage apoptosis (P<0.001) and late-stage apoptosis (P<0.01), and cause obvious reductions in extracellular ATP and intracellular IP3 levels during cisplatin-induced apoptosis (P<0.05).</p><p><b>CONCLUSION</b>Pannexin 1 channels participate in cisplatin-induced apoptosis in I-10 cells possibly through the ATP/IP3 pathway.</p>
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Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article