Role of microRNA-181a in the apoptosis of tubular epithelial cell induced by cisplatin / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 523-526, 2012.
Article
in En
| WPRIM
| ID: wpr-262579
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Cisplatin (DDP) is one of most effective and most commonly used therapeutic agent in treating tumors, it can accumulate in the kidney and lead to acute renal failure. MicroRNA-181a can induce cell apoptosis by suppressing the expression of Bcl-2 family. In the present study, we investigated the role of microRNA-181a in the apoptosis of tubular epithelial cell induced by DDP.</p><p><b>METHODS</b>HK-2 cells were cultured, transfected with microRNA-181a inhibitor for 48 hours, and stimulated with 50 µmol/L cisplatin for 24 hours. MicroRNA-181a expression was analyzed by real time PCR, and cell apoptosis was detected by flow cytometry. Moreover, Bcl-2 and Bcl-2-associated X protein (Bax) expression were measured by Western blotting.</p><p><b>RESULTS</b>MicroRNA-181a expression significantly down-regulated in cells transfected with microRNA-181a inhibitor, compared with that in untransfectd cells (21.19 ± 2.01 vs. 38.87 ± 1.97, P < 0.05). Cell apoptosis induced by DDP significantly decreased in cells transfected with MicroRNA-181a inhibitor. Compared with DDP treated cells alone, Bcl-2 expression strikingly was up-regulated and Bax expression was down-regulated in cells transfected with microRNA-181a inhibitor.</p><p><b>CONCLUSION</b>One pathway of DDP induces apoptosis of tubular epithelial cell by suppressing Bcl-2 expression is achieved by regulating the target gene of MicroRNA-181a.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Transfection
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Cell Line
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Blotting, Western
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Cisplatin
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Apoptosis
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Proto-Oncogene Proteins c-bcl-2
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Reverse Transcriptase Polymerase Chain Reaction
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MicroRNAs
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RNA, Small Interfering
Limits:
Humans
Language:
En
Journal:
Chinese Medical Journal
Year:
2012
Type:
Article