Effect of 25-hydroxyvitamin D(3) on vitamin D receptor expression and distribution in human bronchial epithelial cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 28-31, 2012.
Article
in Zh
| WPRIM
| ID: wpr-265703
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of 25-hydroxyvitamin D(3) on the expression and distribution of vitamin D receptor in normal human bronchial epithelial cells.</p><p><b>METHODS</b>MTT assay was used to assess the viability of human airway epithelial cell line 16HBE following a 24-h exposure to different concentrations of 25-hydroxyvitamin D(3). Real-time quantitative PCR, Western blotting, and immunofluorescence assay were used to observe the expression and distribution of vitamin D receptor in the cells following the exposure.</p><p><b>RESULTS</b>Compared with the control cells, 16HBE cells exposed to different concentrations of 25-hydroxyvitamin D(3) exhibited no significantly increase in the expression or distribution of vitamin D receptor.</p><p><b>CONCLUSION</b>The influence of 25-hydroxyvitamin D(3) on bronchial epithelial cells might be independent of the expression and translocation of vitamin D receptor.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Bronchi
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Calcifediol
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RNA, Messenger
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Cell Line
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Receptors, Calcitriol
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Cell Biology
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Epithelial Cells
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Genetics
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article