HPLC-ELSD determination of triterpenoids and triterpenoid saponins in Ilex pupurea leaves / 药学学报

ABSTRACT

<p><b>AIM</b>To establish an HPLC-ELSD method for the quantification of pedunculoside, ziyuglycoside I and rutundic acid, in the leaves of Ilex purpurea Hassk.</p><p><b>METHODS</b>By optimizing the chromatographic conditions of HPLC and the parameters of ELSD to study the methodology. Column Allsphere ODS-2 (250 mm x 4.6 mm ID, 5 microns), mobile phase methanol-water (5941), flow rate 1.0 mL.min-1, drift tube temperature 59 degrees C, gas flow rate 2.38 L.min-1.</p><p><b>RESULTS</b>The calibration curves were linear in the range of 2.56-25.60 micrograms for pedunculoside, 1.64-16.40 micrograms for ziyuglycoside I and 3.74-37.40 micrograms for rutundic acid. The average recovery of pedunculoside was 96.3%, RSD 1.59% (n = 5), ziyuglycoside I 97.3%, RSD 3.82% (n = 5), rutundic acid 97.7%, RSD 2.11% (n = 5). All of RSDs of the precision were less than 4% (n = 6), and the reproduciblities less than 5% (n = 6).</p><p><b>CONCLUSION</b>The method is simple, accurate, effective and feasible. It can be used for the determination of the contents of pedunculoside, ziyuglycoside I and rutundic acid in leaf of Ilex purpurea Hassk.</p>