Your browser doesn't support javascript.
loading
Characterization of human anti-BAFF scFv-Fc that inhibits the activity of BAFF in vivo / 药学学报
Acta Pharmaceutica Sinica ; (12): 1336-1340, 2012.
Article in Chinese | WPRIM | ID: wpr-274657
ABSTRACT
To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Spleen / Blood / Body Weight / Recombinant Fusion Proteins / Immunoglobulin G / Immunoglobulin M / Immunoglobulin Fc Fragments / B-Lymphocytes / Random Allocation / Cell Differentiation Type of study: Controlled clinical trial Limits: Animals / Female / Humans Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2012 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Spleen / Blood / Body Weight / Recombinant Fusion Proteins / Immunoglobulin G / Immunoglobulin M / Immunoglobulin Fc Fragments / B-Lymphocytes / Random Allocation / Cell Differentiation Type of study: Controlled clinical trial Limits: Animals / Female / Humans Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2012 Type: Article