Characterization of human anti-BAFF scFv-Fc that inhibits the activity of BAFF in vivo / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1336-1340, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-274657
ABSTRACT
To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spleen
/
Blood
/
Body Weight
/
Recombinant Fusion Proteins
/
Immunoglobulin G
/
Immunoglobulin M
/
Immunoglobulin Fc Fragments
/
B-Lymphocytes
/
Random Allocation
/
Cell Differentiation
Type of study:
Controlled clinical trial
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2012
Type:
Article
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