Study of the effect of hepatitis C virus core protein on interferon-induced antiviral genes expression and its mechanisms / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1000-1004, 2007.
Article
in Zh
| WPRIM
| ID: wpr-276172
Responsible library:
WPRO
ABSTRACT
To study the effect of HCV core protein on the interferon-induced antiviral genes expression and its mechanisms. Methods HepG2 cells were transiently transfected with HCV core protein expression plasmid and the blank plasmid respectively. RT-PCR was used to analyze the effect of HCV core protein on PKR and 2'-5'OAS expression. The effect of HCV core protein on ISRE-medicated gene expression was detected by luciferase activity assay. Western-blot assay was performed to observe the change of mRNA and protein levels of SOCS3, STAT1 and p-STAT1 following HCV core expression. In the presence of HCV core protein, the transcription of PKR and 2'-5' OAS are down-regulated. ISRE-medicated reporter gene expression and STAT1 phosphorylation were inhibited. The transcription and expression of SOCS3 were induced compared with blank plasmid-transfected group. In HepG2 cells, HCV core protein can down-regulate the expression of some interferon-induced antiviral genes, which involves the induction of SOCS3 and the inhibition of STAT1 phosphorylation.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Physiology
/
Protein Kinases
/
Transcription, Genetic
/
2',5'-Oligoadenylate Synthetase
/
Tumor Cells, Cultured
/
Transfection
/
Down-Regulation
/
Viral Core Proteins
/
Interferon-alpha
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article