Expression of BMP-4 in stromal cells in vitro derived from human aorta-gonad-mesonephros region / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 772-775, 2007.
Article
in Zh
| WPRIM
| ID: wpr-276825
Responsible library:
WPRO
ABSTRACT
The objective of this study was to investigate the expression of BMP-4 in stromal cells in vitro derived from human aorta-gonad-mesonephros (AGM) region. Stromal cells derived from human AGM region (hAGM S1-S5) and fibroblasts derived from human fetal trunk (hFT) were cultured in vitro. RT-PCR was used to analyze the expression of BMP-4 in hAGM S1-S5 stromal cells at mRNA level. And BMP-4 level was detected in the supernatant of hAGM S1-S5 stromal cells by ELISA assay. hFT cells were used as control group. The results showed that the heterogenous hAGM S1-S5 stromal cells displyed shapes of fibroblast-like and endothelial-like cells. hAGM S1-S5 stromal cells expressed BMP-4 mRNA, but fetal trunk fibroblasts (hFT) did not express BMP-4 mRNA. In the supernatant of hAGM S1-S5 cells, BMP-4 could be detected by ELISA assay ana its levels were statistically higher than that in hFT group (p < 0.05), while there was no significant difference between groups of hAGM S1-S5 (p > 0.05). It is concluded that human AGM-derived stromal cells in vitro express BMP-4, and the establishment of a new culture system based on the feeder cells of AGM stroma would promote the differention of embryonic stem cells into hematopoietic stem cells at a high proportion.
Full text:
1
Index:
WPRIM
Main subject:
Aorta
/
RNA, Messenger
/
Hematopoietic Stem Cells
/
Cells, Cultured
/
Embryology
/
Stromal Cells
/
Cell Biology
/
Bone Morphogenetic Protein 4
/
Gonads
/
Mesonephros
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article