Determination of complanatoside A in semen Astragali complanati by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the determination method for complanatoside A in seeds of Astragalus complanatus.</p><p><b>METHOD</b>An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm.</p><p><b>RESULT</b>The good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.</p><p><b>CONCLUSION</b>The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.</p>