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Comparison of two urinary protein preparation methods: nitrocellulose membrane preservation and acetone precipitation / 生物工程学报
Chinese Journal of Biotechnology ; (12): 982-989, 2014.
Article in Chinese | WPRIM | ID: wpr-279454
Responsible library: WPRO
ABSTRACT
Nitrocellulose membrane based urinary protein preservation method is simple, fast and economic, but its advantage over the traditionally used acetone precipitation method is still unclear. In this work, we prepared urinary proteins by the two methods by LC-MS/MS. Then we used protein spectra counts to assess the reproducibility of the two methods. Proteins identified by the two methods were almost the same in number, spectral count distribution and distribution of coefficients of variation value. In conclusion, nitrocellulose membrane method is generally the same as acetone precipitation method. It can be used for large scale preservation of clinical urine samples.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Acetone / Mass Spectrometry / Urine / Humans / Proteins / Chemistry / Reproducibility of Results / Chromatography, Liquid / Collodion / Tandem Mass Spectrometry Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2014 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Acetone / Mass Spectrometry / Urine / Humans / Proteins / Chemistry / Reproducibility of Results / Chromatography, Liquid / Collodion / Tandem Mass Spectrometry Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2014 Type: Article