Polysaccharides from Scurrula parasitica L. inhibit sarcoma S180 growth in mice / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 381-384, 2010.
Article
in Zh
| WPRIM
| ID: wpr-281012
Responsible library:
WPRO
ABSTRACT
To study the anti-tumor activity of Scurrula parasitica polysaccharides (SP). Water extraction and ethanol precipitation were used to isolate SP from S. parasitica leaf. S180, K562 and HL-60 cell lines proliferation inhibition by SP were detected by MTT assay. The expressions of Ki-67, Cyclin D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP+ SP could not inhibit cancer cell proliferation. SP ip could inhibit the growth of sarcoma S180 in mice, 100 mg x kg(-1) x d(-1). SP ip was the optimal dose on inhibiting S180 growth, with the tumor inhibition rate of 54%. The expression of Ki-67, Cyclin D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP. The result showed that SP could down-regulate the expression of Ki-67, CyclinD1 and Bcl-2 protein, and up-regulate the expression of Bax protein. It indicted that inhibiting cancer cell proliferation and promoting cancer cell apoptosis in vivo maybe one of the anti-cancer mechanisms of SP.
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
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Polysaccharides
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Sarcoma 180
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Drugs, Chinese Herbal
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Immunohistochemistry
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Chemistry
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HL-60 Cells
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Proto-Oncogene Proteins c-bcl-2
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Cyclin D1
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K562 Cells
Limits:
Animals
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Female
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Humans
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Male
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article