Effect of combination of glutamine and mitomycin C on human gastric carcinoma cell strain MGC-803 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 345-349, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-281201
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of combination of glutamine (GLN) and mitomycin C (MMC) on the human gastric carcinoma cell line MGC-803 in vitro.</p><p><b>METHODS</b>The effects of GLN and MMC were measured by MTT assay, and the interaction between the two agents was evaluated by the median-effect principle. Flow cytometry was used for cell cycle analysis.</p><p><b>RESULTS</b>GLN did not significantly stimulate the cell growth in vitro. High-concentration of GLN could inhibit the cell growth. MMC could effectively inhibit the cell growth in a time-dependent manner. The interaction of these two agents showed a weak antagonistic activity (1 < CI < 1.2703). MMC induced remarkable S-phase arrest. Low-dose GLN has limited effect on the S-phase arrest of MMC, while high-dose GLN significantly attenuated the S-phase arrest and lowered the proliferation index of MGC-803 cell.</p><p><b>CONCLUSIONS</b>Combination of GLN and MMC has a a weak and dose-dependent antagonistic activity in the treatment of gastric carcinoma cell line MGC-803. The combination of high-dose MMC and low-dose GLN may achieve better efficacy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Stomach Neoplasms
/
Adenocarcinoma
/
Mitomycin
/
Cell Line, Tumor
/
Dose-Response Relationship, Drug
/
Drug Synergism
/
Glutamine
/
Antibiotics, Antineoplastic
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2006
Type:
Article
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