MicroRNAs can be expressed in cardiomyocyte-like cells differentiated from human mesenchymal stem cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1813-1816, 2007.
Article
in Zh
| WPRIM
| ID: wpr-281534
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of representative heart-specific primary microRNAs (pri-miRNAs) in the cardiomyocyte-like cells differentiated from human mesenchymal stem cells (hMSCs).</p><p><b>METHODS</b>The phenotype of hMSCs isolated was identified by flow cytometry using monoclonal antibodies against FITC-conjugated CD29, CD34, and CD11b. The third-passage hMSCs were induced to differentiate into cardiomyocyte-like cells by 5-azacytidine and indirect coculture with neonatal rat myocytes, respectively. Immunocytochemical analysis was performed to detect the expression of the cardiac-specific proteins, namely cardiac troponin I (cTnI) and sarcomeric alpha-actinin, in the cardiomyocyte-like cells differentiated from hMSCs. RT-PCR and DNA sequencing were used to identify the expression of the 5 representative heart-specific pri-miRNAs.</p><p><b>RESULTS</b>High hMSC marker CD29 expression rate (98.87%) and low hematopoietic cell markers CD34 (5%) and CD11b (0.4%) expression rates were identified in the hMSCs isolated. cTnI and sarcomeric alpha-actinin expression occurred in the hMSCs following induction with the 2 differentiation-inducing methods. miRNA-143 and -181 expressions were induced in the hMSCs by 5-azacytidine and miRNA-143, -181, -206, and -208 expressions were induced by indirect coculture with neonatal rat myocytes, but pri-miRNA-1-2 expression failed to be induced by these two induction methods.</p><p><b>CONCLUSION</b>Expressions of the representative heart-specific pri-miRNAs in different patterns can be induced in cardiomyocyte-like cells differentiated from hMSCs by 5-azacytidine and indirect coculture with neonatal rat myocytes.</p>
Full text:
1
Index:
WPRIM
Main subject:
Actinin
/
Cell Differentiation
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Coculture Techniques
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Troponin I
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Cell Biology
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Myocytes, Cardiac
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MicroRNAs
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Mesenchymal Stem Cells
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Metabolism
Limits:
Animals
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Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article