Generation and analysis of 113 adult stage Schistosoma japonicum (Chinese strain) expressed sequence tags / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1517-1520, 2002.
Article
in English
| WPRIM
| ID: wpr-282151
ABSTRACT
<p><b>OBJECTIVE</b>To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST).</p><p><b>METHODS</b>A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags (ESTs). These were compared against an EMBL-parasites database and GENBANK database by BLASTn and BLASTx.</p><p><b>RESULTS</b>A total of 314 phage clones were randomly selected for generating expressed sequence tags (ESTs). From these clones, 132 EST-quality sequence were obtained. Among these EST-quality sequences, 113 ESTs were successfully submitted to the dbEST at GenBanK. A total of 7.6% of these EST-quality sequences were previously identified sequence of Schistosoma japonicum, while 4.5% were putatively identified sequences of Schistosoma japonicum. A total of 23.5% of these EST-quality sequences were putatively identified sequence of Schistosoma mansoni or other organisms. 57.6% had no matches in the database and were classified as unknown sequences. Most ESTs with the putative protein identified belonged to housekeeping proteins. Information about several interesting genes was found.</p><p><b>CONCLUSION</b>Partial cDNA sequencing to generate expressed sequence tags (ESTs) has the potential to rapidly and economically increase our knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Schistosoma japonicum
/
Molecular Sequence Data
/
Base Sequence
/
Gene Library
/
Chemistry
/
DNA, Complementary
/
Expressed Sequence Tags
/
Genetics
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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