The change of telomere protein in BEAS-2B malignant transformation cell induced by coal tar pitch smoke extracts / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>By testing the changes of telomere binding protein in malignant transformation BEAS-2B cells induced by coal tar pitch smoke extracts, to study the role of protection of telomeres 1 (POT1), telomeric repeat binding factor 1 (TRF1) and TRF2 in tumorgenesis that contact with coal tar pitch.</p><p><b>METHODS</b>The BEAS-2B cells were induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, the protein expression variations were determined by cell culture overslip of immunohistochemical methods.</p><p><b>RESULTS</b>In malignant transformation cells, the mRNA expression level (POT1 0.63 ± 0.04, TRF1 0.36 ± 0.01) and the protein expression level (POT1 0.36 ± 0.05, TRF1 0.09 ± 0.03) of POT1 and TRF1 was statistically significant decreased compared to that of BEAS-2B group (mRNA POT1 1.00 ± 0.04, TRF1 1.01 ± 0.16; protein POT1 0.55 ± 0.07, TRF1 0.27 ± 0.07) and DMSO group (mRNA POT1 0.89 ± 0.12, TRF1 0.90 ± 0.08; protein POT1 0.55 ± 0.10, TRF1 0.26 ± 0.04) (P < 0.05); mRNA expression level (1.45 ± 0.07) and the protein expression level (0.88 ± 0.06) of TRF2 was increased compared to that of BEAS-2B group (mRNA 1.00 ± 0.07, protein 0.48 ± 0.06) and DMSO group (mRNA 1.00 ± 0.06, protein 0.50 ± 0.06) (P < 0.05).</p><p><b>CONCLUSION</b>The change of gene and protein expression level in POT1, TRF1, and TRF2 involved in the process that evolved into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.</p>