Extract of Zuojin Pill (characters: see text) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 837-845, 2015.
Article
in En
| WPRIM
| ID: wpr-287180
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of water extract of Zuojin Pill ([characters see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism.</p><p><b>METHODS</b>Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9.</p><p><b>RESULTS</b>ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation.</p><p><b>CONCLUSIONS</b>ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.</p>
Key words
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Drugs, Chinese Herbal
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Cell Survival
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Blotting, Western
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Reactive Oxygen Species
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Apoptosis
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Colorimetry
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Comet Assay
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Cell Line, Tumor
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Mitochondrial Membranes
Limits:
Humans
Language:
En
Journal:
Chinese journal of integrative medicine
Year:
2015
Type:
Article