Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 205-208, 2011.
Article
in Zh
| WPRIM
| ID: wpr-289398
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the bioaffinity between 8 bufadienolides(Bu) and tumor cells and analyze the correlation between the bioaffinity and the anti-tumor activities of Bu.</p><p><b>METHOD</b>Mix and cultivate the chloroform extract of Chansu and MGC-803. Measure the content of 8 Bu in supernatant and cells using HPLC and calculate their affinity rate.</p><p><b>RESULT</b>The coefficient correlation between the decrease of Bu in cell supernatant after affinity and its MGC-803 restrictive activities, and between the cotent percentage of the free Bu in free cells with its MGC-803 restrictive activities, and between the difference between the decrease and the percentage and its MGC-803 restrictive activities is r = 0.82 (P < 0.05), r = -0.04 and r = 0.83 (P < 0.05) respectively.</p><p><b>CONCLUSION</b>Eight Bu have different levels of affinity with MGC-803 which correlate with their anti-tumor activities.</p>
Full text:
1
Index:
WPRIM
Main subject:
Anura
/
Pharmacology
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Bufanolides
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Chemistry
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Chromatography, High Pressure Liquid
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Cell Line, Tumor
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Drug Therapy
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Amphibian Venoms
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Methods
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Neoplasms
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article