Your browser doesn't support javascript.
loading
Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities / 中国中药杂志
Article in Zh | WPRIM | ID: wpr-289398
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the bioaffinity between 8 bufadienolides(Bu) and tumor cells and analyze the correlation between the bioaffinity and the anti-tumor activities of Bu.</p><p><b>METHOD</b>Mix and cultivate the chloroform extract of Chansu and MGC-803. Measure the content of 8 Bu in supernatant and cells using HPLC and calculate their affinity rate.</p><p><b>RESULT</b>The coefficient correlation between the decrease of Bu in cell supernatant after affinity and its MGC-803 restrictive activities, and between the cotent percentage of the free Bu in free cells with its MGC-803 restrictive activities, and between the difference between the decrease and the percentage and its MGC-803 restrictive activities is r = 0.82 (P < 0.05), r = -0.04 and r = 0.83 (P < 0.05) respectively.</p><p><b>CONCLUSION</b>Eight Bu have different levels of affinity with MGC-803 which correlate with their anti-tumor activities.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Anura / Pharmacology / Bufanolides / Chemistry / Chromatography, High Pressure Liquid / Cell Line, Tumor / Drug Therapy / Amphibian Venoms / Methods / Neoplasms Limits: Animals Language: Zh Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article
Full text: 1 Index: WPRIM Main subject: Anura / Pharmacology / Bufanolides / Chemistry / Chromatography, High Pressure Liquid / Cell Line, Tumor / Drug Therapy / Amphibian Venoms / Methods / Neoplasms Limits: Animals Language: Zh Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article