Induced differentiation of human cord blood mesenchymal stem/progenitor cells into cardiomyocyte-like cells in vitro / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 154-157, 2003.
Article
in En
| WPRIM
| ID: wpr-290488
Responsible library:
WPRO
ABSTRACT
The feasibility of using cord blood mesenchymal stem/progenitor cells (CB-MSPCs) to regenerate cardiomyocytes and the optimal inducing conditions were investigated. The CB mononuclear cells were cultured in low serum DMEM medium to produce an adherent layer. After expansion, the adherent cells were added into cardiomyocyte inducing medium supplemented with 5-azacytidine. Cardiogenic specific contractile protein troponin T staining was performed to identify the cardiomyocyte-like cells. The results showed that the frequency of CB-MSPCs clones in CB mononuclear cells was 0.5 x 10(-6) and about 1.3 x 10(7)-fold expansion was achieved within 20 sub-cultivation. After cardiogenic induction, 70% CB-MSPCs was differentiated into cardiomyocyte-like cells. It was indicated that low serum culture could expand CB-MSPCs extensively and the expanded CB-MSPCs could be induced to differentiate into cardiomyocyte-like cells in high efficiency.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Azacitidine
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Cell Differentiation
/
Cells, Cultured
/
Fluorescent Antibody Technique
/
Culture Media, Conditioned
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Troponin T
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Cell Biology
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Myocytes, Cardiac
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Fetal Blood
Limits:
Female
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Humans
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Male
/
Pregnancy
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2003
Type:
Article