Effects of apoptosis of Tca8113 cells induced by adriamycin on telomerase and telomere repeat binding factor proteins / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 654-655, 2006.
Article
in Zh
| WPRIM
| ID: wpr-293019
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of telomerase and telomere repeat binding factors (TRF) in apoptosis.</p><p><b>METHODS</b>The proliferative activity of Tca8113 cells was assessed by methyl thiazolyl tetrazolium (MTT) assay. After Tca8113 cells were treated with adriamycin at 5 mg/L, apoptotic morphology was observed under microscope with Giemsa staining and apoptosis examined by flow cytometry; analysis of telomerase activity was performed by TRAP-enzyme-linked immunosorbent assay; expression and expression level of TRF proteins were detected with immunohistochemical staining and immunofluorescence label assay, respectively.</p><p><b>RESULTS</b>After Tca8113 cells were treated with adriamycin at 5 mg/L for 5 days and 7 days, the cells apoptosis was found. Telomerase activity dropped in time-dependent manner. Expression of TRF proteins appeared in nucleus of the cells. No statistical difference in expression levels of TRF was observed between the treated and untreated cells.</p><p><b>CONCLUSIONS</b>Tca8113 cells apoptosis induced by adriamycin decreased telomerase activity, but did not influence the expression level of TRF proteins.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Tongue Neoplasms
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Carcinoma, Squamous Cell
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Doxorubicin
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Apoptosis
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Telomerase
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Telomere-Binding Proteins
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Cell Line, Tumor
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Stomatology
Year:
2006
Type:
Article