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Determination of epimedin C and icariin in Herba Epimedii by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1025-1027, 2003.
Article in Chinese | WPRIM | ID: wpr-293738
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC method for the determination of epimedin C and icariin in Herba Epimedii.</p><p><b>METHOD</b>Chromatographic conditions Hypersil BDS-C18 column, acetonitrice and water containing 0.05% phosphoric acid as gradient eluents, G1315A photodiode array detector at 272 nm.</p><p><b>RESULT</b>The average recovery rate of epimedin C was 99.7%, RSD 1.5% (n = 9), icariin 102.5%, RSD 1.1% (n=9).</p><p><b>CONCLUSION</b>The method is reliable, stable and well repeatable. It provides the useful information for quality evaluation.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Species Specificity / Flavonoids / Drugs, Chinese Herbal / Chemistry / China / Classification / Ecosystem / Epimedium Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Species Specificity / Flavonoids / Drugs, Chinese Herbal / Chemistry / China / Classification / Ecosystem / Epimedium Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2003 Type: Article