Effect of total glucosides of paeony on nuclear factor-kappaB activation in rat peritoneal macrophages / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 669-671, 2008.
Article
in Zh
| WPRIM
| ID: wpr-295461
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of total glucosides of paeony (TGP) on lipopolysaccharides (LPS)-induced nuclear factor-kappaB (NF-kappaB) activation in macrophages.</p><p><b>METHOD</b>Rat peritoneal macrophages were pre-treated with TGP for 2 h and stimulated with LPS for 20 min or 0.5 h. Inhibitory kappaBalpha (IkappaBalpha) protein in the cytoplasm and NF-kappaB p65 protein in the nuclear were analyzed by western blot. Further, DNA binding activity of NF-kappaB complex was detected.</p><p><b>RESULT</b>TGP enhanced the amounts of IkappaBalpha protein in the cytoplasm and decreased the amounts of NF-kappaB p65 protein in the nuclear of LPS-induced macrophages. TGP also inhibited the LPS-mediated DNA binding activity of NF-kappaB complex in macrophages.</p><p><b>CONCLUSION</b>TGP can inhibit LPS-induced NF-kappaB activation in macrophages through arresting IKBalpha protein degradation, NF-kappaB p65 protein nuclear translocation and DNA binding activity of NF-kappaB complex.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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DNA
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Cell Nucleus
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Chemistry
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NF-kappa B
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Macrophages, Peritoneal
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Protein Transport
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Paeonia
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Cell Biology
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Cytoplasm
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article