Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 594-598, 2016.
Article
in En
| WPRIM
| ID: wpr-296563
Responsible library:
WPRO
ABSTRACT
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 μg/mL cyclopamine group was 68.9% lower than that of blank control group (P<0.05). Hedgehog signaling pathway inhibition decreased palatal fusion in organ culture, probably owing to downregulation of Smo and Gli1 proteins.
Full text:
1
Index:
WPRIM
Main subject:
Palate
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Signal Transduction
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Embryology
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RNA, Small Interfering
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Kruppel-Like Transcription Factors
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Hedgehog Proteins
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Zinc Finger Protein Gli3
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Zinc Finger Protein Gli2
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Genetics
/
Metabolism
Limits:
Animals
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2016
Type:
Article